Peroxynitrite-Based Luminol Luminescence of Macrophages and Enhancement of the Signal

Initially, it was thought that macrophages could not produce light, but using luminol we and others

showed that macrophages can produce luminol-dependent chemiluminescence (LDCL). Macrophages produce much less light in the unstimulated state than do neutrophils (~l/100). ln fact, macrophages produce light without the addition of luminol as do neutrophils, but without luminol it takes many more cells to observe CL.