ABSTRACT
Operons in Gram-Negative Bacteria...............................................519 40.5 Expression of the
lux
Operons in Gram-Positive Bacteria ................................................519 40.6 Correlative Cell Culture Assays..........................................................................................520 40.7 Monitoring Bioluminescent Gram-Negative Bacteria in Live Mice..................................520 40.8 Generating and
in Vivo
Monitoring of Bioluminescent Gram-Positive Bacteria ..............521 40.9 Summary and Conclusions .................................................................................................523 Acknowledgments ..........................................................................................................................523 References ......................................................................................................................................523
Animal model experiments involving bacterial diseases typically employ assays such as enumerating pathogenic cells by counting colony-forming units (CFU) or identifying and quantifying their proteins or nucleic acids. These assays are labor intensive and require the removal of tissues. Consequently, there is a loss of the contextual influences that were present in the living animal. Rapid
