ABSTRACT
M
Tris-HCl; BSA 1.25 mg / mL
•
25 m
M
CoCl
1 µL
•
Labeled nucleotide
50 µCi
➫
≈
1000 µCi / mmol (
α
S-dNTP)
•
TdT 25 units/µL
1 µL
➫
Terminal deoxytransferase
•
Sterile distilled water
to 10 µL
b. Incubate.
60 min at 37˚C
2. Precipitation
a. Add 10 mg/mL tRNA.
