ABSTRACT
This step must obey the same demands as for biochemical homogenates while preserving sample morphology and cytology.
All types of biological material can be used, including:
• Organ
➫
For histological sections • Biopsy
➫
For histological sections • Cell culture
➫
See
Figure 2.1. – Plated monolayer
➫
On presterilized glass coverslips – In suspension
➫
In culture flask: •After cytocentrifugation •After pellet formation, freezing, and sectioning
➫
Cells stacked onto a glass slide with cytocentrifuge (necessary in case of a low-density cell suspension).
