ABSTRACT
Although the risk of contamination in solvents is minimal, the baths must be changed regularly.
b. Rehydrate: • Alcohol 100
°
5 min
• Alcohol 95
°
5 min
• Alcohol 70
°
5 min
• NaCl 9‰
5 min
➫
Na
ions maintain the nucleic acids
in situ
.
Although the risk of contamination in solvents is minimal, the baths must be changed regularly.
b. Rehydrate: • Alcohol 100
°
5 min
• Alcohol 95
°
5 min
• Alcohol 70
°
5 min
• NaCl 9‰
5 min
➫
Na
ions maintain the nucleic acids
in situ
.