ABSTRACT

Since the beginning of science, mankind has always been interested in looking at deep structures of natural and synthetic materials to understand and analyze their properties and behavior under different types of interaction. After the development of the first conventional light microscope (magnification, about 200×) by Antoni van Leeuwenhoek in the fifteenth century, a race to improve the magnification and quality of the images began, resulting in the improvement of lens design, lens configuration, and new methods of sample preparation. Several advances were made in the field of optics by the German optician Ernst Abbe in 1873, resulting in one of his most important conclusions: the relationship between the wavelength of light and the resolving power of a microscope, given by

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