ABSTRACT
The human genome is frequently assaulted by endogenous and exogenous sources.1 To gain insights into the biological consequences of DNA damage, it is important to ask how effectively a DNA lesion inhibits DNA replication and how frequently it causes mutations. These questions are often addressed by rst constructing oligodeoxyribonucleotides (ODNs) harboring a structurally de ned DNA lesion. The ODNs can then be treated with puri ed DNA polymerases and the resulting replication products can be assessed by using the traditional gel electrophoresis techniques.
