ABSTRACT
Barley Chromosomes ................................................................143 11.4 Single-Copy Gene Detection on Human Chromosomes Using
SNOM/AFM .......................................................................................... 145 11.5 Multicolor FISH Detection Using SNOM/AFM ....................................147 11.6 Discussion and Conclusion .................................................................... 150 Acknowledgment .............................................................................................. 151 References ......................................................................................................... 152
Fluorescence in situ hybridization (FISH) is a technique used to visualize defined nucleic acid sequences in cellular preparations by hybridization of complementary probe sequences, and is widely applied to the detection of a specific gene on the chromosome by fluorescence light microscopy. However, the resolution of conventional FISH by fluorescence light microscopy is in practice at the level of about half of a micrometer, due to optical limitations. Thus, there is need for a novel technique in order to visualize a specific gene on a chromosome at the nanometer scale.
