ABSTRACT
Scanning (SEM) and transmission (TEM) electron microscopy provide valuable tools for assessing morphological changes found in various mouse mutations. The theory behind the preparation of samples for both types of EM is the same: samples are collected and fixed (if necessary) in such a way that the morphology of the finished product is as close to lifelike as possible. Consequently, the speed with which the samples are collected and fixed in a carefully selected fixative and buffering system all determine the quality of the final result. Care and attention to detail provide high-quality results that support other studies.
