ABSTRACT
High-performance liquid chromatography (HPLC) is an instrumental chromatography configuration in which the mobile phase is a liquid. The discussion of the principles of instrumental chromatography presented in Chapter 11 (Sections 11.8.3 and 11.8.4) provides the basic background for this technique. A liquid mobile phase is made to move through a column containing the stationary phase. A mixture of compounds injected ahead of the column separates as the compounds pass through. The mixture components are then detected electronically one at a time as they elute from the column, resulting in the recording of the instrumental chromatogram by the data collection system (refer to Figures 11.6 and 11.15).
