ABSTRACT
Introduction .............................................................................................................. 58 Phage Display Technology ....................................................................................... 58 Site-Directed Mutagenesis and Alanine Scanning ................................................... 59 The Shotgun Scanning Method ................................................................................60 Examples of Shotgun Scanning ............................................................................... 61
Shotgun Scanning of hGH ..................................................................................64 Homolog and Serine Scanning .......................................................................64 Shotgun Scanning of a High Affinity hGH Variant ........................................64 Assessing Intramolecular Cooperativity ........................................................66 Mapping Binding Sites in a Comprehensive Manner .....................................66
