ABSTRACT
The retention of ionogenic analytes in RP-HPLC is obviously dependent on the eluent pH. The pH value is an important optimization parameter because it controls the extent of ionization of the solute and hence the magnitude of electrostatic interactions. Varying the acidity of the mobile phase can lead to extreme changes in selectivity. Many interdependent parameters can be modulated in IPC and their optimization requires a theory-driven procedure.
