ABSTRACT
With the rapid developments in molecular biology tools, genome sequencing and bioinformatics it has become very easy to manipulate enzyme levels and properties in metabolic reaction networks, with the aim to achieve desirable changes in product formation rates by cells and microorganisms. For many interesting products the stoichiometry of their synthesis pathways is known, including their links to central metabolism. Much less known is the eect of changes in enzyme/transporter levels (or changes in their kinetic properties) in the product pathway or in central metabolism on the product ux.
