2Chapter 0 Extraction and Purication of DNA from Marine Sediments Suitable for the Analysis of Prokaryotic Diversity

Wash Solution 1: 50 mM Tris-HCl, pH 8.3; 200 mM NaCl; 5 mM Na2EDTA; 0.05% Triton X-100.

Wash Solution 2: 50 mM Tris-HCl, pH 8.3; 200 mM NaCl; 5 mM Na2EDTA. Wash Solution 3: 10 mM Tris-HCl, pH 8.3; 0.1 mM Na2EDTA. Ultrapure water: Reagent-grade water, previously autoclaved, ltered (through 0.2 µm) and

stored in sterile 2-mL Eppendorf tubes at 4°C. UltraClean Soil DNA Isolation Kit (MoBio, catalog #12800-100). The kit contains: Bead

Solution tubes, solution S1, solution IRS, solution S2, solution S3, solution S4, and solution S5. Moreover, spin columns are also included for DNA purication.