Tetrasodium pyrophosphate solution: Prepare a stock solution of 500 mM tetrasodium pyrophosphate (SIGMA) in reagent-grade water preltered onto 0.2 µm lters (Whatman Puradisc).

SYBR Green I working solution: Original SYBR Green I stock (Invitrogen) is in concentrated form (10,000×), dissolved in DMS, and should be stored in the dark at −20°C. Before dilution, the stock solution must be de-frozen. Since the uorochrome and the DMS have different cryoscopic points, be sure that both are completely melted. An ideal working solution of SYBR Green I has to be diluted 20 folds, in order to obtain 500× nal concentration.