ABSTRACT
Isolation Procedure. The plant samples collected were cut into square pieces ~1 cm2 and washed twice with sterilized artificial seawater at 50% salt concentration. The cut samples were then placed aseptically on the diluted GPY agar medium on which Psychrobacter phenylpyruvicus had been grown. The plates were sealed with parafilm and incubated at room temperature. For the next 7 d, we observed the plates daily with the inverted microscope. Due to their specific spindle shape and gliding movement, the labyrinthulids were easily recognizable. The square pieces of the agar medium, including an ectoplasmic network of labyrinthulids cells, were subcultured in a new agar medium with Psychrobacter phenylpyruvicus; this procedure was repeated 2-3 times to obtain a monoxenic culture with an isolated labyrinthulid and Psychrobacter phenylpyruvicus.
