ABSTRACT
Separation of Lipid Classes. Lipid classes were separated according to Singleton and Stikeleather (4) on a silica column (25 × 0.46 cm, 5 µm, Luna, silica (2), Phenomenex, Torrance, CA) with a linear gradient of 2-propanol/hexane (4:3, vol/vol) to 2-propanol/hexane/water (4:3:0.75, by vol) in 20 min, then isocratically for 20 min. A prepacked silica saturator column (3 × 0. 46 cm, 15-25 µm, Phenomenex) was installed between the pump and injector to saturate the mobile phase with silica before it reached the column.
