ABSTRACT
Introduction The integration of chromatographic and spectroscopic analytical techniques now represents the mainstream in advanced analysis of natural compounds. Usually, this approach can be described as a two-step procedure. In the first step, the mixture of compounds is separated by a suitable technique such as gas chromatography (GC) or high-performance liquid chromatography (HPLC). Second, a specific detection method, for example, mass spectrometry (MS), is used to obtain structural information about individual substances. In an ideal case, this approach makes it possible to identify simultaneously the individual compounds in the sample, or at least estimate their chemical structures.
