ABSTRACT
Creatine kinase isoenzymes have been extensively used in the clinicallaboratory for the differential diagnosis of myocardial infarction, as well as in the assessment of head injury [1,2], neoplastic diseases [3-8], muscular dystrophy [3,9] , and neurological diseases [10, 11]. A variety of techniques have been utilized to separate these polypeptides, including immunochemical assays with antisera prepared to the Band M subunits of the molecule [6,9,12-17]. Polyclonal and monoclonal antibodies generated to the Band M polypeptides have also been used in studying tissue extracts [18] with radioimmunoassays as well as immunohistochemical assays [19] of various tissues. The B polypeptide of this enzyme has been shown to be present in a variety of tissues [18,19]. In addition, increased serum levels of the BB isoenzyme have been associated with a variety of malignant diseases [3,4,5,20-23].
