ABSTRACT
Protein Data Bank-https://www.rcsb.org/pdb/ Prosthetic Groups and Metal Ions in Protein Active Sites Database (Promise)—
https://bioinf.leeds.ac.uk/promise Metalloproteins Database-https://metallo.scripps.edu
Magnesium is found as an important component of a broad variety of enzymes, as compared to most other metals except calcium. The high concentration of magnesium in most biological systems likely has contributed to its selection in the function of many enzymes, where it can assume different roles, e.g., stabilizing high-energy sub strate conformations, polarizing substrates for nucleophilic attack, orienting sub strates for reaction, and so forth. It is also important to note that Mn2+ has often been used in place of Mg2+ for many in vitro enzymatic and structural studies, whereas in vivo Mg2+ is often the biologically relevant metal cation; thus, one must be aware of the possible distinction between the in vitro and in vivo situations.
