ABSTRACT

Examples of such types of objects are autoradiographic grains, cell nuclei, cell bodies, microorganisms, and colonies of microorganisms. There are also nonbiological applications, for example, in astronomy (counting of stars and galaxies) and geology. In the life sciences, the applications are most frequently found in the field of the neuroscience, where grains are the most often counted type of object. Originally, counting strategies, especially the algorithms, have mostly been developed for autoradiographic grains, 1-6 but minor modifications often make them applicable for other object types. In addition, counting procedures for other round particles have been published.7-12 Due to the historical development, this chapter focuses especially on the methods of computerized autoradiographic grain counting.