ABSTRACT
SPE yields excellent protein recovery rates much higher compared to other conventional methods assayed, such as concentration by rotatory vacuum evaporator, precipitation with S04(NH4) 2 or lyophilisation (6,7). In addition, SPE allows
RP-HPLC I
Fraction V MeOH post -elut
18 h tap water MWCO 4000 D
Table 1
I RP-HPL 0 0 r c 3: z
processing several samples concurrently, to automate the procedure and so the cost is reduced. Errors due to manipulating are avoided because SPE provides concentrated extracts ready to be directly injected into the analytical system and thereby an on-line method may be configured.
