ABSTRACT
A common test for the allelopathic potential of a plant in vitro is to determine the effects of its aqueous extract on the seed germination and subsequent radicle growth. The seed is an ideal tool because it is a dispersal unit of life, consisting of three genetically different parts: seed coat, endosperm and an embryo able to sprout out as a new plant, if exposed to the right conditions (Evenari, 1980). This methodical paper deals with the morphological and cytological responses of two different seeds viz., radish (Raphanus sativus L. 'Saxa') and purslane (Portulaca oleracea L.), sown with rue (Ruta graveolens L.) extract. We have made efforts to simplify the basic microscopic techniques for a beginner.
