ABSTRACT
Historically, the main use of affinity chromatography has been in the isolation of biological agents. Examples given throughout this book include the use of affinity columns for the isolation of enzymes, antibodies, and recombinant proteins. This has made affinity chromatography an important preparative tool in biochemistry and biotechnology. But the successful use of affinity chromatography for large-scale isolations requires that several aspects of this method be considered and characterized. This chapter examines many of these factors and discusses their roles in affinity separations.
