ABSTRACT
Taylor and Nickless [1] have described a paired-ion high performance liquid chromatographic technique for the separation of mixtures of linear alkylbenzene sulphonates and p-sulphophenylcarboxylate salts,
( -C-C-C-C-C-COOH)
in river waters. Partially biodegraded linear alkylbenzene sulphonate was analysed by the same method. Structural information on measurable intermediates formed was provided by stopped-flow ultraviolet spectra, comparison of retention behaviour with that of standards and analysis of collected fractions. Samples (1.5L) were concentrated for analysis by acidification with sulphuric acid to pH2, followed by passage through a column containing 20mL of XAD-4 resin at a flow rate of 7ml min _ 1 . Compounds retained by the resin were eluted with 3 x 25mL portions of methanol and the combined eluates evaporated to dryness then up to 2ul.
