ABSTRACT
IV. DERIVATIZA TION METHODS Another strategy to prevent the interaction between proteins and the surface of fused-silica capillaries involves the chemical bonding of neutral and/or hydrophilic coatings via silane derivatization. This approach appears attractive for preparing a uniform and stable coating in order to control the EOF and reduce protein adsorption. Different chemistry routes were developed to attach the reactive groups to the silica surface with siloxane bonds (Si-0-Si-C) or with direct Si-C linkages. In addition, various hydrophilic polymers were proposed for the direct covalent attachment to the inner capillary wall. Another approach well adapted to the EC of proteins implies the in situ copolymerization (inside the capillary tubing) of the monomer chemically bonded to the surface with a suitable monomer.
