ABSTRACT
Harsch and Vouros [54] coupled CZE in a PVP matrix to an ion trap mass spectrometer for the identification of DNA adducts formed between synthetic short oligonucleotides and 2-(N-acetoxy-N-acetylamino)fluorene (AAAF). CZE in the PVP matrix very successfully separated the different oligonucleotide-AAF adducts from each other and from the unmodified oligonucleotides. The acquisition of MS and MS/MS spectra allowed for the determination of the base composition and the sequence of the adducts. Based on these results, one can conclude that this approach provides a novel tool for the investigation of the sequence specificity of carcinogen DNA-adduct modifications [54].
