ABSTRACT
Tzong-Hsien Lee* and Marie-Isabel Aguilar Department of Biochemistry and Molecular Biology, Monash University, Victoria, Australia
I. INTRODUCTION 176 IL BIOMEMBRANE-MODIFIED SOFT-GEL CHROMATO-
GRAPHIC SUPPORTS 177 A. Entrapment of Whole Cells 177 B. Immobilization of Liposomes and Proteoliposomes 178
III. BIOMEMBRANE-MODIFIED SILICA-BASED CHROMATOGRAPHIC SUPPORTS 182 A. Covalently Attached Phospholipid Monolayers 183 B. Adsorbed Monolayers and Bilayers 186
IV. APPLICATIONS 187 A. Protein Purification 187 B. Bioaffinity Measurements 188 C. Drug Partition Measurements 189 D. Peptide-and Protein-Membrane Interactions 190
V. CONCLUSIONS AND FUTURE DIRECTIONS 196 VI. REFERENCES 197
I. INTRODUCTION High-performance liquid chromatography (HPLC) is now firmly established as the premier technique for the analysis and purification of a wide range of molecules. In particular, HPLC in its various modes has become the central technique in the characterization of peptides and proteins and has therefore played a critical role in the rapid advances in the biological and biomedical sciences over the last 10 years [1].
