ABSTRACT
A library of genomic DNA is a set of recombinant vectors each containing a different part of the genome of the species being studied. The principle consists of the following: (1) isolating the genomic DNA from the species being studied; (2) digesting that DNA using a restriction enzyme that allows the liberation of restriction fragments of a length compatible with the chosen vector;1 (3) cloning the fragments in a cloning vector; (4) integrating the recombinant vectors in a microorganism in order to multiply them. The phage vectors (see Profile 7) are better adapted to cloning of a given genomic sequence, while the cosmids (see Profile 7) and YACs or BACs (see Profile 8) are chosen to characterize a genome. The library obtained is thus made up of millions of recombinant clones.
