. Modified nucleosides explicitly involved in tRNA identity for aminoacylation

Glutamine system (with class lb GlnRS): Treatment of E. coli tRNA^111 (with s2!-^) with the thiol reagent BrCN produces an important decrease in glutamine acceptance, while tRNA2an (with C 3 4 ) and other tRNAs not possessing a reactive thiol group in their anticodon were not affected by the BrCN treatment.83 This suggests a determinant role o f s2! ^ in glutamine identity.