ABSTRACT

Primers are small DNA strands (from 15 to 30 base pairs long) that are complementary to a specific spot in a DNA molecule. They are needed for DNA replication to take place. In molecular biology, primers are used for a DNA amplification chain reaction called PCR (Polymerase Chain Reaction). PCR primers have their own characteristics like: specific melting temperature, primer length, need to avoid self-complementarity and other parameters.1