ABSTRACT
In this case we have a text file full of PCR primers. These primers were obtained from different sources, so their Tm was calculated under different programs and conditions. A researcher may want to uniform the criteria of Tm of his set of primers. The first version of the program will output the file formated as a csv
(comma separated value). This kind of file could be opened with a spreadsheet or with a custom made program. The second version will output the file as an Excel spreadsheet (xls). Proposed steps to get the melting temperatures of a set of primers:
1. Read the input file line by line.
