ABSTRACT
Biological events in cells are caused by specic interactions among a wide variety of biomolecules, including proteins, nucleotides, chemicals, and so on. Chemical biology is focused on elucidatingthe mechanism and network of biological reactions by using chemical compounds. Several chemical compounds show specic inhibition or activation against target proteins, mediated by a specic binding to the functional domain of proteins. Therefore, the identication of target proteins for chemical compounds provides us with valuable information about biological and biochemical reactions, and also anew insight for developing therapeutic agents by an analysis of structureactivityrelationship. Despite the importance of identifying target molecules, some difculties are encountered in aconventional afnity purication system, due to their high nonspecic binding and weakness of physical properties of the carrier. To resolve such problems, we have developed anovel afnity nanoparticle for high-performance purication. In Section 9.2, we introduce the construction and the properties of our afnity particles.
