ABSTRACT
The effects of temperature as a variable in high-performance liquid chromatography (HPLC) are pervasive, inuencing every aspect of the experiment. Temperature changes solvent viscosity and solute diffusion rates, which has led to a resurgence in interest in elevated temperature separations for a faster analysis. The increased backpressure required for sub-2 μm columns has been a major factor in this trend. Very large increases in speed, by an order of magnitude or more, have been made possible by the combination of ultrahigh-pressure liquid chromatography (UHPLC), narrow bore columns, and moderately elevated temperatures.
