ABSTRACT
Affinity chromatography is a liquid chromatographic technique that uses a specic binding agent for the purication or analysis of sample components [1-6]. The retention of a solute or target substance in this method is based on selective and reversible interactions that occur in many biological systems, such as the binding of an enzyme with a substrate or a hormone with its receptor. These interactions are used in afnity chromatography by immobilizing one of a pair of interacting molecules onto a solid support and placing it into a column. The immobilized molecule is referred to as the affinity ligand and makes up the stationary phase of the afnity column [2,6].
