The analysis of enzymes in detergents is based on the standardmethodology of protein identification and enzyme activity determination. This type of analysis seems relatively easy since the number of relevant candidates is limited, especially when compared to number and type of proteases found intracellular or extracellular of single and wellknown microorganisms like, for example, Bacillus subtilis. The task sometimes becomes tedious and complicated due to the fact that qualitative and quantitative determinations have to be performed in a complex matrix, consisting of substances that are, to a significant degree, able to interact with enzyme activity and stability.