ABSTRACT
Anticoagulant Study Reference
EDTA Measurement of angiogenic cytokines (e.g., VEGF) in blood plasma. EDTA causes variable platelet activation with release of cytokines; cytokines were not released when citrate-theophyllineadenosine-dipyridamole (CTAD) was used as an anticoagulant
EDTA; citrate The level of hyaluronidase activity in EDTA plasma is comparable to the level of hyaluronidase activity in serum; the level of hyaluronidase activity in citrated plasma is lower than the level in serum
EDTA; citrate VEGF concentration was similar in EDTA-PGE1-theophylline plasma and CTAD plasma; VEGF was signi€cantly higher in citrated plasma
Citrate Soluble CD40 ligand (sCD40L) concentration was evaluated in citrated plasma and CTAD plasma. Sample processed in the cold had low concentrations and there was no difference between the two plasmas in either normal subjects or acute coronary syndrome (ACS) patients; for samples processed at room temperature, sCD40L was elevated in ACS patients
EDTA; citrate The concentration of soluble CD40 ligand (sCD40L) was higher in EDTA plasma than citrated plasma
EDTA; citrate Soluble thrombomodulin was unstable in EDTA but was stable in acidi€ed citrated plasma
EDTA; citrate; heparin
MALDI/TOF/MS of low-molecular-weight proteins (prefractionation with C8 or Cu-IMAC). Difference in proteomes depends on anticoagulant with largest difference between EDTA and heparin or citrate
Heparin; citrate; EDTA
No difference in IL-6 levels as a function of type of anticoagulant 59
Heparin; citrate; EDTA
A review on the effect of various anticoagulants on the activity of circulating matrix metalloproteinases in plasma
EDTA; heparin A small difference in the immunoreactivity of β-C-telopeptide between potassium EDTA plasma and lithium heparin plasma
EDTA, heparin, citrate
Difference in low molecular weight peptides from plasma as determined by MALDI/TOF/MS. Peptides obtained from plasma by €ltration through 3 kDa nominal mw cutoff €lter. Addition of protease inhibitors prevented degradation
Heparin plasma, citrated plasma, EDTA plasma, serum
Emphasis on the preparation of samples for the analysis of peptides. Analysis by RP-HPLC/MS.a Pattern depends on anticoagulant choice. The use of EDTA or citrated plasma recommended for study of low-molecular-weight proteome. Heparin plasma pattern was markedly different from either EDTA or citrated plasma
a Samples prepared by ultra€ltration of plasma or serum after denatured in guanidine (50 kDa cutoff €lter) were separated on RP-HPLC (C5) and ef†uent fractions analyzed by MALDI-MS.
