ABSTRACT

Free radicals are highly reactive molecules with unpaired electrons that can be generated in vivo during metabolic processes. Reactive oxygen species (ROS) represent the most important class of free radicals generated in living systems. It is accepted that ROS play different roles in vivo. Some are positive and are related

10.1 Introduction .................................................................................................. 257 10.2 Biosensors for Determination of the Total Phenol Content .......................... 258

10.2.1 Polyphenol Oxidase-Based Biosensors ............................................. 259 10.2.1.1 Tyrosinase-Based Biosensors ............................................. 259 10.2.1.2 Laccase-Based Biosensors ................................................. 261

10.2.2 Peroxidase-Based Biosensors ........................................................... 262 10.3 Biosensors for Determination of the Reactive Oxygen Species (ROS)

Scavenging Capacity ..................................................................................... 263 10.3.1 Cyt c-Based Biosensors ....................................................................264 10.3.2 Superoxide Dismutase-Based Biosensors .........................................266 10.3.3 DNA-Based Biosensors .................................................................... 267

10.4 Conclusion ....................................................................................................268 Acknowledgment ...................................................................................................269 References ..............................................................................................................269