ABSTRACT
Notes: (1) Labeled dsDNA must be denatured before it is used for hybridization. This can be done by boiling the DNA for 10 min then quickly chilling it on ice for 3 min. (2) After hybridization, the probe contained in the hybridization buffer can be stored at −20°C and reused up to four times. (3) The yield of the labeled DNA can be estimated by dot blotting of serial dilutions of commercially labeled control DNA and labeled sample DNA on a nylon membrane „lter. After hybridization and detection, the spot intensities of the control and the labeled DNA can be compared.
