Protein design remains a challenge both for academic and industrial researchers looking to improve upon the products of natural evolution. Producing a protein with a particular function de novo is usually beyond our capabilities, and even redesigning an existing protein to enhance speci’c characteristics is challenging. One area of protein engineering in which success has been demonstrated is in the design of proteins exhibiting improved thermal stability. Proteins might be desired that have either longer functional lifetimes at some high operating temperature or improved activity at lower temperatures at the expense of thermal stability.