ABSTRACT
Pounder et al. [5] described a real-time PCR with SYBR green DNA-binding dye and amplicon melting temperature analysis for fungal detection using pan-fungal primers ITS1 forward (5′-TCCGTAGGTGAACCTGCGG-3′) and ITS4 reverse (5′-TCCTCCGCTTATTGATATGC-3′) [7]. The identity of the fungi is veri–ed by subsequent sequence analysis.
