ABSTRACT
A variety of GCs is available in the market. All GCs operate under the same principle. Headspace GC consists of a long column packed with inert material. An aliquot of the sample of blood or serum is put in a vial that is sealed with a rubber septum or stopper and is heated. e vapors from the vial are injected automatically into the column. Vaporized compounds are moved through the column by inert gases. e compounds are separated while moving through the column. At the end of the column, a detector detects the compounds by a signal generated and gives out a peak on a chart that rolls at speciˆed speed. e compounds are identiˆed by the retention times. A known standard compound of known quantity mixed with a sample generates a peak. is standard peak area is compared with the area of the compound in question. e peak areas of the standard are compared with the peak area of the compound in question and are used for quantization.
