ABSTRACT
Introduction Cephalosporins consist of a fused β-lactam-∆3-dihydrothiazine two-ring system, known as 7-aminocephalosporanic acids (7-ACAs) and vary in their side chain substituents at C3 (R2) and C7 (acylamido, R1). e chemical structure of the studied cephalosporins in this work is shown in Table 1. ey are used for treatment of infection caused by both gram-negative and gram-positive bacteria [1, 2]. A wide variety of analytical methods have been reported for determination of cephalosporins in pure form, in pharmaceutical preparations, and in biological uids. ese methods include spectrophotometry [2-5], atomic absorption spectrophotometry [6], uorometry [7-12], liquid chromatography [13-20], Micellar electrokinetic capillary chromatography [21, 22], chemiluminescence [23-28], potentiometric [29, 30], and polarographic [31-34] methods. Kinetic spectrophotometric methods became of great interest in chemical and pharmaceutical analyses [35]. e literature is still poor in analytical procedure based on kinetics, especially for determination of drug in commercial dosage forms. We aimed to improve on the current methods by employing the kinetic colorimetric oxidation of cephalosporins to increase selectivity, avoid interference of colored and/or turbidity background of samples and consequently determination of low concentration of the cited drugs as possible.
