ABSTRACT
Background Skeletal muscle myogenesis involves numerous steps including the proliferation, migration and fusion of myoblasts to form myotubes; the onset of myobrillargenesis, and the maturation and hypertrophy of muscle bres [1,2]. Myogenesis in teleost sh has several unique features compared to mammals, including the production of myotubes throughout much of adult life [3]. e in vitro culture of sh myogenic cells is an attractive system for studying the formation and dierentiation of myotubes and examining the eects of various regulatory molecules on gene expression under precisely controlled conditions [4,5]. Furthermore, since traditional gene “knockouts” are unavailable in sh, cell culture provides a viable alternative for functional assays.
