ABSTRACT

Figure 15.1 Microscopic examination using hemocytometer to count sperm in the ejaculate (a) and (b). Note an automatic counter at the bottom of (b) to count simultaneously viable and non-viable sperm, leukocytes, debris and other cell contaminants. (c) Micropipetting for evaluation, counting and storage in cryopreservation straws for sperm and eggs

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problems regarding the fertility of the subject arise, using both natural and AI.Therefore, it is necessary to develop methods of enchancing the proportion of viable cells present when AI is to be attempted using a donor semen.