ABSTRACT
Surface plasmon resonance (SPR) and biosensor technologies for biospecic interaction analysis (BIA)1 enable monitoring of DNA:DNA and DNA:RNA hybridization in real time.2 In the BIAcore biosensor system (Figure 24.1a), plane-polarized light is reected from the gold-coated sensor chip (Figure 24.1b), where the molecular interactions take place.2 SPR in the gold layer results in the extinction of the reected light at a specic angle. This angle (SPR angle) varies with the refractive
24.1 Introduction .......................................................................................................................... 339 24.2 Materials and Methods ......................................................................................................... 341
24.2.1 Primer, Molecular Probes, and Target Oligonucleotides .......................................... 341 24.2.2 DNA Templates and PCR Conditions....................................................................... 342 24.2.3 SPR-Based BIA ......................................................................................................... 342
24.2.3.1 Protocol A: Determination of the Optimal Conditions for Hybridization, Including Detection of Point Mutations.............................344
