The issue of protein stability was first explained on a fundamental level by Wu in 1931 [1] where he proposed a theory on protein denaturation after publishing twelve papers on his experimental observations of protein denaturation [2]. In 1954, Lumry and Eyring published a seminal paper [3] entitled “Conformation Changes of Proteins” that laid the groundwork for what we know today about protein structure, folding, stability, and aggregation.