ABSTRACT
In the second chapter of this book, you were introduced to the basic theory of chromatography. Here in this chapter, we shall look more specifically at the modes of separation used in HPLC. Already, the concept of a mobile phase and a stationary phase has been established. Here, we further discuss this basic concept and explain the chemical nature of the stationary and mobile phases and how varying these, as well as considering the analytes we have, has an effect on the separations that can be carried out.
