ABSTRACT
The first application of high-performance liquid chromatography to plant pigments was by Evans et al [1] and others [21-24, 28, 29, 66-69] who separated phaeophytins a and b on Corasil II with a mobile phase consisting of a 1:5 (v/v) mixture of ethyl acetate and light petroleum. Eskins et al [2] have employed two 0.62m columns of C18-Porasil B for preparative separation of plant pigments by means of a programmed stepwise elution with methanol-water-ether. However, the method is of little value for routine application because of the time required and also because the chlorophyll degradation products, other than phaeophytin, are not separated.
