ABSTRACT
The main objective of gene therapy is a successful in vivo transfer of genetic materials to the targeted tissues. Virus-based gene carriers involve potential risks of immunogenicity, and chromosomal insertion of viral genome. In contrast, nonviral vectors such as lipoplexes are preferable for their many advantages, including lower toxicity, nonimmunogenicity, and nononcogenicity, greater nucleic acid packaging capacity, and easier and cheaper preparation. However, from the therapeutic point of view, a level of transfection suffers low efciency when lipoplexes are used as gene delivery vectors. The establishment of a correlation between transfection efciency and various characteristics of the lipoplexes requires thus in-depth research (Madhusudhana Rao and Gopal 2006, Li and Huang 2006, Zuhorn et al. 2007, Donkuru et al. 2010).
